Detection of prothrombin mutation G20210A among acute lymphoblastic leukemia Sudanese patients at Khartoum state

Abstract

Abstract:
Background: Cancer is one of the most common causes of disease-related mortality in children in
many countries. The most prevalent kind of leukemia in children is acute lymphoblastic leukemia
(ALL). Is a hematological malignancy that originates from the abnormal proliferation of B-type or
T-lymphocytes in the bone marrow. The prothrombin mutation gene (G20210A) increases the risk
of thrombosis, and it is the second most common hereditable thrombophilia defect found in patients
with venous thromboembolic disorder (VTE). Objectives: This study aimed to find out how to
detect the prothrombin mutation gene (G20210A) in children with acute lymphoblastic leukemia.
DNA was extracted from whole blood by using iNtRON biotechnology (G-DEX TM IIb), and the
mutation analysis for prothrombin (G20210A) was used by conventional thermal cycle PCR
(sensoquest) and agarose gel to amplify the PCR product, which was visualized under UV light by
the gel documentation system Methods: This is a case-control study conducted in Khartoum state at
the Radiation Isotope Centre Khartoum, which included one hundred, 50 children with acute
lymphoblastic leukemia (case group), male and female, aged 3–20 years, and 50 healthy children of
matched age and gender. From each participant, 3 ml of venous blood was collected in Ethylene
Diamine Tetra Acetic (EDTA) vacutainers. Results: The study result revealed that out of 50
participants, no prothrombin mutation (G20210A) was detected among ALL patients. The study
also showed no mutation among the control group. Conclusion: The study concluded that there is no
association between the prothrombin mutation (G20210A) gene and Sudanese acute lymphoblastic
leukemia patients.
Keywords: Prothrombin, Mutation, G20210A, ALL, leukemia