The Effect of Solvents to Extract Phenol and Phenol Related Compounds Contents and Some Biological Screening of Salvia officinalis

Abstract

Saliva officinalis (Lamiaceae) herbal plant collected from Khartoum local market. This study has 
three aims, to assess the efficacy of three aqueous organic solvents to extract the phenol and phenol 
derivative metabolites using the maceration method, to assess the antifungal and anticancer of 
Saliva officinalis aqueous extracts using Yeast – Based bioassay against three mutant yeast strains 
Saccharomyces cerevisiae, and to assess the antioxidant activity of Saliva officinalis extracts using 
two methods: DPPH radical scavenging assay, and TLC Autography technique. The results 
revealed that the 70% methanol has high total phenols (318.35mg GAE/g) and total flavonoids 
(189.38mg QE/g) while the 70% acetone extract was revealed high total tannin content (218.34 mg 
TAE/g). The 70% acetone extract revealed activity against the three mutant yeast tested with 
inhibition zone diameter more than 12 mm at concentration 5mg/ml and 10mg/ml respectively. 
While the other extracts were exhibited no activity against the three mutant strains tested. All 
extracts were reflected anti-Candida activity and the maximum IZD was exhibited by the 
70%ethanol extract (30mm). All extracts were free-radical inhibitors but 70% methanol extract was 
more potent (0.068mg/ml) than the other extracts